LOCUS       40924_31665       13739 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pMpGWB305 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13739)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T.
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha
  JOURNAL   PLoS ONE 10 (9), E0138876 (2015)
  PUBMED    26406247
REFERENCE   2  (bases 1 to 13739)
  AUTHORS   Nishihama R, Ishizaki K, Kohchi T.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUN-2015) Contact:Ryuichi Nishihama Kyoto University, 
            Graduate School of Biostudies; Kitashirakawa-oiwake-cho, Sakyo-ku, 
            Kyoto, Kyoto 606-8502, Japan URL :http://www.lif.kyoto-u.ac.jp/e/
REFERENCE   3  (bases 1 to 13739)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 13739)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2015"; volume: "10"; issue: "9"; pages: "E0138876"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (01-JUN-2015) Contact:Ryuichi Nishihama Kyoto University, Graduate 
            School of Biostudies"; volume: " Kitashirakawa-oiwake-cho, Sakyo-ku,
            Kyoto, Kyoto 606-8502, Japan URL :http"; pages: 
            "//www.lif.kyoto-u.ac.jp/e"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..13739
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(54..78)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             1178..1894
                     /codon_start=1
                     /label=Citrine
                     /note="enhanced variant of YFP (Heikal et al., 2001)"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     protein_bind    1904..2028
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        2065..2095
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             2149..2805
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             3150..3452
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(3496..3620)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      3646..3898
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3932..3948)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3956..3972
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3980..4010)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4025..4046)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     polyA_signal    complement(4131..4306)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(4314..6350)
                     /codon_start=1
                     /gene="mALS"
                     /product="acetolactate synthase"
                     /label=mALS
                     /note="from Marchantia polymorpha that contains triple 
                     amino-acid subustitutions (P207S/R208S/W582L)"
                     /protein_id="BAS49891.1"
                     /translation="MASISGCAAVPLSIASLEKVQPSARSCCKGAKELKVRSGFFSGAA
                     LRGHAYNTSRKTERSLEVKAQAVSEKIQSAVDVSGPRLSSGKSLVAPTPSDFQSRYKDD
                     EPRKGADILVEALEREGIRTVFAYPGGASMEIHQALTRSSSIRNILCRHEQGEIFAAEG
                     YAKSSGRVGVCIATSGPGATNLVTGLADALLDSVPLVAITGQVSSRMIGTDAFQETPIV
                     EVTRSITKHNYLVMNIDDIPRVIREAFFLAASGRPGPVLVDIPKDVQQQMAIPDWSQPM
                     QLKGYLQRLPRPPEPTLIEQIIRLISSSKKPVVYSGGGCNHASEELREFVELTGIPITS
                     TLMGLGAYPESDPLSLKMLGMHGTVYANYAVDQADLLLAFGVRFDDRVTGKLETFASRS
                     SIVHIDIDPAEIGKNKQPHVSMCADVKPALSEINALLKSQKRTFDFSNWVAELEEQKQK
                     WPMKFPVFEDAIAPQSAIQTLYELTGGNAIISTGVGQHQMWAAQWFKYNEPRRWLTSGG
                     LGAMGFGLPSALGAAATQPDIPVIDIDGDGSFVMNVQELATIFVEKLPVKIMILNNQHL
                     GMVVQLEDRFYKANRAHTYLGDPDHEEQIYPDFCTVADGFKVPNARVVKKDELRAAIQT
                     MLDSPGPYLLDVIVPHQEHVLPMIPGGASFKEIITEGDGRTIY"
     gene            complement(4314..6350)
                     /gene="mALS"
                     /label=mALS
     promoter        complement(6390..7066)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     misc_feature    complement(7261..7285)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7806..8594
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8843..9431
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(9617..9757)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(10101..10295)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(10364..11434)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11866..12492)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag aatgagcatg gtgagcaagg gcgaggagct
     1201 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     1261 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     1321 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tcggctacgg
     1381 cctgatgtgc ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     1441 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     1501 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     1561 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     1621 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     1681 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     1741 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagctacc agtccgccct
     1801 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     1861 cgggatcact ctcggcatgg acgagctgta caaggctgtt atcacaagtt tgtacaaaaa
     1921 agctgaacga gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa
     1981 aaaacagact acataatact gtaaaacaca acatatccag tcactatggc ggcccctcga
     2041 ggggccgcat taggcacccc aggctttaca ctttatgctt ccggctcgta taatgtgtgg
     2101 attttgagtt aggatccggc gagattttca ggagctaagg aagctaaaat ggagaaaaaa
     2161 atcactggat ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca
     2221 tttcagtcag ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt
     2281 ttaaagaccg taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc
     2341 cgcctgatga atgctcatcc ggaattccgt atggcaatga aagacggtga gctggtgata
     2401 tgggatagtg ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg
     2461 ctctggagtg aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg
     2521 gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc
     2581 gtctcagcca atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac
     2641 aacttcttcg cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg
     2701 atgccgctgg cgattcaggt tcatcatgcc gtctgtgatg gcttccatgt cggcagaatg
     2761 cttaatgaat tacaacagta ctgcgatgag tggcagggcg gggcgtaaac gcgtggatcc
     2821 ggcttactaa aagccagata acagtatgcg tatttgcgcg ctgatttttg cggtataaga
     2881 atatatactg atatgtatac ccgaagtatg tcaaaaagag gtgtgctatg aagcagcgta
     2941 ttacagtgac agttgacagc gacagctatc agttgctcaa ggcatatatg atgtcaatat
     3001 ctccggtctg gtaagcacaa ccatgcagaa tgaagcccgt cgtctgcgtg ccgaacgctg
     3061 gaaagcggaa aatcaggaag ggatggctga ggtcgcccgg tttattgaaa tgaacggctc
     3121 ttttgctgac gagaacaggg actggtgaaa tgcagtttaa ggtttacacc tataaaagag
     3181 agagccgtta tcgtctgttt gtggatgtac agagtgatat tattgacacg cccgggcgac
     3241 ggatggtgat ccccctggcc agtgcacgtc tgctgtcaga taaagtctcc cgtgaacttt
     3301 acccggtggt gcatatcggg gatgaaagct ggcgcatgat gaccaccgat atggccagtg
     3361 tgccggtctc cgttatcggg gaagaagtgg ctgatctcag ccaccgcgaa aatgacatca
     3421 aaaacgccat taacctgatg ttctggggaa tataaatgtc aggctccctt atacacagcc
     3481 agtctgcagg tcgaccatag tgactggata tgttgtgttt tacagtatta tgtagtctgt
     3541 tttttatgca aaatctaatt taatatattg atatttatat cattttacgt ttctcgttca
     3601 gctttcttgt acaaagtggt gataacctag agctcgaatt tccccgatcg ttcaaacatt
     3661 tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa
     3721 tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg
     3781 agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa
     3841 atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt actagatcgg
     3901 gaattggttc cggaaccaat tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     3961 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     4021 cctaatgagt gagctaactc acattaggct gaattaggcg cgcctatttc tgaattcaac
     4081 acattgcgga cgtttttaat gtactgaatt aacgccgaat taattcgggg gatctggatt
     4141 ttagtactgg attttggttt taggaattag aaattttatt gatagaagta ttttacaaat
     4201 acaaatacat actaagggtt tcttatatgc tcaacacatg agcgaaaccc tataggaacc
     4261 ctaaattccc ttatctggga actactcaca cattattatg gagaaactcg aagttagtat
     4321 atagtccttc catcaccctc tgtgataatc tccttaaaac ttgctccccc aggaatcata
     4381 ggcagaacgt gctcctggtg gggaactatc acatccagta aataaggacc gggagaatct
     4441 agcatagttt gaattgctgc tcttagttca tccttcttca ccacacgcgc atttggcacc
     4501 ttgaaaccgt ctgcaactgt acagaagtcg gggtagatct gctcctcgtg atctggatca
     4561 cctaagtaag tatgtgctct gttggccttg tagaatctat cctccaattg tacgaccatt
     4621 cctagatgtt ggttgtttaa gatcatgatc ttcacgggta acttctccac aaaaattgta
     4681 gccaactcct gtacgttcat cacaaagcta ccatcgccat cgatgtcgat cacaggaata
     4741 tctggttgag ttgcggcagc ccccaaagca gaaggaagtc cgaatcccat ggctccaaga
     4801 cctccagatg tcaaccaacg ccgcggttcg ttgtacttga accactgggc tgcccacatt
     4861 tgatgttggc caacaccagt agatatgatg gcatttccac cagtgagttc gtacagcgtc
     4921 tgtatggcag attgaggagc aatggcatcc tcgaatacgg gaaacttcat tggccatttc
     4981 tgcttttgtt cttctagttc tgccacccag ttggagaagt cgaaagtcct cttttgactc
     5041 ttcaagaggg cattgatttc acttagcgca ggtttcacat cagcacacat ggacacatgg
     5101 ggctgtttgt tctttccaat ttcagcaggg tcaatatcga tatgaacgat gctggacctg
     5161 ctagcaaaag tctccagttt accagtcact cgatcgtcga aacgcactcc aaatgccaac
     5221 aacagatctg cttggtccac ggcgtagttg gcgtaaacag ttccatgcat tccaagcatt
     5281 ttcaaactta atggatcaga ctcgggatac gctcccaaac ccattaaagt cgatgtgata
     5341 ggaattccgg taagctcgac aaattccctc aactcttcgc tggcgtgatt gcaacctcct
     5401 ccagaataca ctacaggctt cttagaacta gaaatgagtc ttataatctg ctcgattaac
     5461 gttggttccg gtggccgagg cagtcgttgt aagtagccct tcagttgcat tggctgagac
     5521 caatctggaa tagccatttg ctgttgcacg tccttgggaa tatctaccaa gactgggccc
     5581 ggcctacctg aagctgccaa aaagaaagcc tccctgatta cgcgagggat gtcatcaatg
     5641 ttcataacaa ggtaattgtg cttggtgatc gatctcgtaa cctccacaat tggagtttct
     5701 tgaaaagcat cggtaccaat catcctactg gatacttgac ctgtaatggc cactagtggc
     5761 acactgtcca agagggcatc agcgagcccg gtaacaagat tggtcgctcc aggaccggat
     5821 gtggctatac aaacccctac tcgtccagac gatttagcat aaccttcagc agcgaaaatc
     5881 tccccctgct cgtggcgaca gagaatgttg cggatgctgc tggaacgtgt aagagcctgg
     5941 tgaatctcca tagacgctcc gccgggataa gcgaacaccg ttctaatccc ttctcgctca
     6001 agagcctcca ccaaaatgtc ggctcctttg cggggctcgt catccttgta gcgagactga
     6061 aagtcggacg gggttggtgc aacgagtgac ttcccgctgc taagccgtgg tccactcaca
     6121 tcgactgcag actggatctt ctcgctaaca gcttgggctt tcacttccag tgaacgctca
     6181 gtcttccgag aggtgttgta tgcatgtccc ctcagagcgg ctcccgagaa gaaaccactc
     6241 ctcaccttta gctctttagc tcccttgcaa caagaccttg ccgatggctg caccttctcc
     6301 aggctcgcta tgctcaaagg cacagctgcg catccactaa ttgacgccat gctcgagaga
     6361 gatagatttg tagagagaga ctggtgattt cagcgtgtcc tctccaaatg aaatgaactt
     6421 ccttatatag aggaagggtc ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca
     6481 gtggagatat cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc
     6541 acgatgctcc tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga ggcatcttga
     6601 acgatagcct ttcctttatc gcaattatgg catttgtagg tgccaccttc cttttctact
     6661 gtccttttga tgaagtgaca gatagctggg caatggaatc cgaggaggtt tcccgatatt
     6721 accctttgtt gaaaagtctc aatagccctt tggtcttctg agactgtatc tttgatattc
     6781 ttggagtaga cgagagtgtc gtgctccacc atgttcacat caatccactt gctttgaaga
     6841 cgtggttgga acgtcttctt tttccacgat gctcctcgtg ggtgggggtc catctttggg
     6901 accactgtcg gcagaggcat cttgaacgat agcctttcct ttatcgcaat gatggcattt
     6961 gtaggtgcca ccttcctttt ctactgtcct tttgatgaag tgacagatag ctgggcaatg
     7021 gaatccgagg aggtttcccg atattaccct ttgttgaaaa gtctcaatag ccctttggtc
     7081 ttctgagact gtatctttga tattcttgga gtagacgaga gtgtcgtgct ccaccatgtt
     7141 ggcaagctgc tctagccaat acgcaaaccg aattcagaaa taaattcagc ctaattcggc
     7201 gttaattcag tacattaaaa acgtccgcaa tgtgttatta agttgtctaa gcgtcaattt
     7261 gtttacacca caatatatcc tgccaccagc cagccaacag ctccccgacc ggcagctcgg
     7321 cacaaaatca ccactcgata caggcagccc atcagtccgg gacggcgtca gcgggagagc
     7381 cgttgtaagg cggcagactt tgctcatgtt accgatgcta ttcggaagaa cggcaactaa
     7441 gctgccgggt ttgaaacacg gatgatctcg cggagggtag catgttgatt gtaacgatga
     7501 cagagcgttg ctgcctgtga tcaattcggg cacgaaccca gtggacataa gcctgttcgg
     7561 ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg
     7621 aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg
     7681 gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt
     7741 tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa
     7801 acatcatggg ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg
     7861 tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg
     7921 atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg
     7981 atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag
     8041 agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt
     8101 ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg
     8161 caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa
     8221 gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg
     8281 aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact
     8341 gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa
     8401 ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc
     8461 agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct
     8521 tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca
     8581 aggtagtcgg caaataatgt ctagctagaa attcgttcaa gccgacgccg cttcgcggcg
     8641 cggcttaact caagcgttag atgcactaag cacataattg ctcacagcca aactatcagg
     8701 tcaagtctgc ttttattatt tttaagcgtg cataataagc cctacacaaa ttgggagata
     8761 tatcatgcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     8821 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     8881 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     8941 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     9001 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     9061 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     9121 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     9181 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     9241 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     9301 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     9361 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     9421 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     9481 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     9541 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     9601 aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     9661 accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata
     9721 cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc
     9781 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     9841 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcaggg
     9901 tgccttgatg tgggcgccgg cggtcgagtg gcgacggcgc ggcttgtccg cgccctggta
     9961 gattgcctgg ccgtaggcca gccatttttg agcggccagc ggccgcgata ggccgacgcg
    10021 aagcggcggg gcgtagggag cgcagcgacc gaagggtagg cgctttttgc agctcttcgg
    10081 ctgtgcgctg gccagacagt tatgcacagg ccaggcgggt tttaagagtt ttaataagtt
    10141 ttaaagagtt ttaggcggaa aaatcgcctt ttttctcttt tatatcagtc acttacatgt
    10201 gtgaccggtt cccaatgtac ggctttgggt tcccaatgta cgggttccgg ttcccaatgt
    10261 acggctttgg gttcccaatg tacgtgctat ccacaggaaa gagacctttt cgaccttttt
    10321 cccctgctag ggcaatttgc cctagcatct gctccgtaca ttaggaaccg gcggatgctt
    10381 cgccctcgat caggttgcgg tagcgcatga ctaggatcgg gccagcctgc cccgcctcct
    10441 ccttcaaatc gtactccggc aggtcatttg acccgatcag cttgcgcacg gtgaaacaga
    10501 acttcttgaa ctctccggcg ctgccactgc gttcgtagat cgtcttgaac aaccatctgg
    10561 cttctgcctt gcctgcggcg cggcgtgcca ggcggtagag aaaacggccg atgccgggat
    10621 cgatcaaaaa gtaatcgggg tgaaccgtca gcacgtccgg gttcttgcct tctgtgatct
    10681 cgcggtacat ccaatcagct agctcgatct cgatgtactc cggccgcccg gtttcgctct
    10741 ttacgatctt gtagcggcta atcaaggctt caccctcgga taccgtcacc aggcggccgt
    10801 tcttggcctt cttcgtacgc tgcatggcaa cgtgcgtggt gtttaaccga atgcaggttt
    10861 ctaccaggtc gtctttctgc tttccgccat cggctcgccg gcagaacttg agtacgtccg
    10921 caacgtgtgg acggaacacg cggccgggct tgtctccctt cccttcccgg tatcggttca
    10981 tggattcggt tagatgggaa accgccatca gtaccaggtc gtaatcccac acactggcca
    11041 tgccggccgg ccctgcggaa acctctacgt gcccgtctgg aagctcgtag cggatcacct
    11101 cgccagctcg tcggtcacgc ttcgacagac ggaaaacggc cacgtccatg atgctgcgac
    11161 tatcgcgggt gcccacgtca tagagcatcg gaacgaaaaa atctggttgc tcgtcgccct
    11221 tgggcggctt cctaatcgac ggcgcaccgg ctgccggcgg ttgccgggat tctttgcgga
    11281 ttcgatcagc ggccgcttgc cacgattcac cggggcgtgc ttctgcctcg atgcgttgcc
    11341 gctgggcggc ctgcgcggcc ttcaacttct ccaccaggtc atcacccagc gccgcgccga
    11401 tttgtaccgg gccggatggt ttgcgaccgc tcacgccgat tcctcgggct tgggggttcc
    11461 agtgccattg cagggccggc agacaaccca gccgcttacg cctggccaac cgcccgttcc
    11521 tccacacatg gggcattcca cggcgtcggt gcctggttgt tcttgatttt ccatgccgcc
    11581 tcctttagcc gctaaaattc atctactcat ttattcattt gctcatttac tctggtagct
    11641 gcgcgatgta ttcagatagc agctcggtaa tggtcttgcc ttggcgtacc gcgtacatct
    11701 tcagcttggt gtgatcctcc gccggcaact gaaagttgac ccgcttcatg gctggcgtgt
    11761 ctgccaggct ggccaacgtt gcagccttgc tgctgcgtgc gctcggacgg ccggcactta
    11821 gcgtgtttgt gcttttgctc attttctctt tacctcatta actcaaatga gttttgattt
    11881 aatttcagcg gccagcgcct ggacctcgcg ggcagcgtcg ccctcgggtt ctgattcaag
    11941 aacggttgtg ccggcggcgg cagtgcctgg gtagctcacg cgctgcgtga tacgggactc
    12001 aagaatgggc agctcgtacc cggccagcgc ctcggcaacc tcaccgccga tgcgcgtgcc
    12061 tttgatcgcc cgcgacacga caaaggccgc ttgtagcctt ccatccgtga cctcaatgcg
    12121 ctgcttaacc agctccacca ggtcggcggt ggcccatatg tcgtaagggc ttggctgcac
    12181 cggaatcagc acgaagtcgg ctgccttgat cgcggacaca gccaagtccg ccgcctgggg
    12241 cgctccgtcg atcactacga agtcgcgccg gccgatggcc ttcacgtcgc ggtcaatcgt
    12301 cgggcggtcg atgccgacaa cggttagcgg ttgatcttcc cgcacggccg cccaatcgcg
    12361 ggcactgccc tggggatcgg aatcgactaa cagaacatcg gccccggcga gttgcagggc
    12421 gcgggctaga tgggttgcga tggtcgtctt gcctgacccg cctttctggt taagtacagc
    12481 gataaccttc atgcgttccc cttgcgtatt tgtttattta ctcatcgcat catatacgca
    12541 gcgaccgcat gacgcaagct gttttactca aatacacatc acctttttag acggcggcgc
    12601 tcggtttctt cagcggccaa gctggccggc caggccgcca gcttggcatc agacaaaccg
    12661 gccaggattt catgcagccg cacggttgag acgtgcgcgg gcggctcgaa cacgtacccg
    12721 gccgcgatca tctccgcctc gatctcttcg gtaatgaaaa acggttcgtc ctggccgtcc
    12781 tggtgcggtt tcatgcttgt tcctcttggc gttcattctc ggcggccgcc agggcgtcgg
    12841 cctcggtcaa tgcgtcctca cggaaggcac cgcgccgcct ggcctcggtg ggcgtcactt
    12901 cctcgctgcg ctcaagtgcg cggtacaggg tcgagcgatg cacgccaagc agtgcagccg
    12961 cctctttcac ggtgcggcct tcctggtcga tcagctcgcg ggcgtgcgcg atctgtgccg
    13021 gggtgagggt agggcggggg ccaaacttca cgcctcgggc cttggcggcc tcgcgcccgc
    13081 tccgggtgcg gtcgatgatt agggaacgct cgaactcggc aatgccggcg aacacggtca
    13141 acaccatgcg gccggccggc gtggtggtgt cggcccacgg ctctgccagg ctacgcaggc
    13201 ccgcgccggc ctcctggatg cgctcggcaa tgtccagtag gtcgcgggtg ctgcgggcca
    13261 ggcggtctag cctggtcact gtcacaacgt cgccagggcg taggtggtca agcatcctgg
    13321 ccagctccgg gcggtcgcgc ctggtgccgg tgatcttctc ggaaaacagc ttggtgcagc
    13381 cggccgcgtg cagttcggcc cgttggttgg tcaagtcctg gtcgtcggtg ctgacgcggg
    13441 catagcccag caggccagcg gcggcgctct tgttcatggc gtaatgtctc cggttctagt
    13501 cgcaagtatt ctactttatg cgactaaaac acgcgacaag aaaacgccag gaaaagggca
    13561 gggcggcagc ctgtcgcgta acttaggact tgtgcgacat gtcgttttca gaagacggct
    13621 gcactgaacg tcagaagccg actgcactat agcagcggag gggttggatc aaagtacttt
    13681 gatcccgagg ggaaccctgt ggttggcatg cacatacaaa tggacgaacg gataaacct
//
